About this product

Product Identifiers

PublisherSpringer

ISBN-109811654840

ISBN-139789811654848

eBay Product ID (ePID)3050404895

Product Key Features

Book TitleMixed-Mode Chromatography : Principles, Methods, and Applications

Number of PagesXiii, 482 Pages

LanguageEnglish

Publication Year2021

TopicPharmacy, Chemistry / Analytic, Chemistry / Organic

IllustratorYes

GenreScience, Medical

AuthorQian-Hong Wan

FormatHardcover

Dimensions

Item Weight35.1 Oz

Item Length9.3 in

Item Width6.1 in

Additional Product Features

Reviews"The present book represents a key reference in the field of mixed-mode chromatography ... . The ten chapters are well balanced, with many fundamental concepts on retention mechanisms as well as more applicative ones, which give the reader a complete overview of the state-of-the-art and future perspectives in this emerging, yet very promising field. Latest innovations related to fabrication of mixed-mode materials are also covered, with particular attention to computer-aided approaches to optimise their chemical structure and properties." (Alberto Cavazzini, Analytical and Bioanalytical Chemistry, Vol. 415, 2023)

Number of Volumes1 vol.

Table Of ContentChapter 1.Historical Perspectives.- Chapter 2.Theoretical Background.- Chapter 3.Modes of Chromatographic Separation.- Chapter 4.Chromatographic Matrices .- Chapter 5.Ligand Design.- Chapter 6.Retention Mechanisms.- Chapter 7.Stationary Phases.- Chapter 8.Mobile Phases and Elution.- Chapter 9.Method Development.- Chapter 10.Applications in Biopharmaceutical Processing. .

SynopsisThe book is about the technology and application of Mixed-mode chromatography (MMC). Unlike conventional single-mode HPLC, which resolves the analytes primarily based on their ionic or hydrophobic properties, MMC employs multifunctional stationary phases to exploit at least two modes of interactions (i.e., ionic and hydrophobic) with the analytes and as such often provides resolution that far exceeds that observed with a single-mode process. Over the past two decades, MMC has developed into an important analytical and purification tool in a number of applications in pharmaceutical and biotechnology industries. The technique has been used widely for the analyses of nucleic acids, amino acids, peptides, proteins, glycoproteins, carbohydrates, antibiotics, vaccines, and other products. The purpose of this book is to present a comprehensive survey of mixed-mode chromatography and is intended as a reference guide for graduate students and experienced scientists in pharmaceutical and biotechnology disciplines wishing to gain a deep understanding of this continuously evolving technology.

LC Classification NumberRS400-431