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A Laboratory Guide to RNA Isolation Analysis and Synthesis by Paul A. KRIEG HTF

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Condition:
Very Good
Used textbook, still in excellent, like new condition. Text is clean, no marks, highlights. Tiny ... Read moreabout condition
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eBay item number:391460266826
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Item specifics

Condition
Very Good
A book that has been read but is in excellent condition. No obvious damage to the cover, with the dust jacket included for hard covers. No missing or damaged pages, no creases or tears, and no underlining/highlighting of text or writing in the margins. May be very minimal identifying marks on the inside cover. Very minimal wear and tear. See all condition definitionsopens in a new window or tab
Seller Notes
“Used textbook, still in excellent, like new condition. Text is clean, no marks, highlights. Tiny ...
ISBN
9780471125365
Category

About this product

Product Identifiers

Publisher
Wiley & Sons, Incorporated, John
ISBN-10
0471125369
ISBN-13
9780471125365
eBay Product ID (ePID)
291723

Product Key Features

Number of Pages
456 Pages
Publication Name
Laboratory Guide to Rna : Isolation, Analysis, and Synthesis
Language
English
Publication Year
1996
Subject
Life Sciences / Cell Biology, Life Sciences / Biochemistry
Type
Textbook
Author
Paul A. Krieg
Subject Area
Science
Format
Trade Paperback

Dimensions

Item Height
0.8 in
Item Weight
28.8 Oz
Item Length
9.8 in
Item Width
6.9 in

Additional Product Features

Intended Audience
Scholarly & Professional
LCCN
96-019936
TitleLeading
A
Dewey Edition
20
Illustrated
Yes
Dewey Decimal
574.87/3283
Table Of Content
Partial table of contents: RNA Isolation from Cells and Tissues (M. Mehra). Isolation of RNA from Plant Tissue (T. Wilkins & L. Smart). Analysis of Polysomal RNA (O. Meyuhas, et al.). Primer-Extension Analysis of RNA (C. George & J. Kadonaga). In Vitro Translation of Cellular and Synthetic RNA (A. Kaminski & N. Standart). Quantitation of RNA Transcripts Using RT-PCR (F. Ferré et al.). Analysis of Messenger RNA by Differential Display (P. Liang). A New Methods for Full-Length cDNA Cloning By PCR (A. Chenchik, et al.). Whole-Mount In Situ Hybridization to Mouse Embryos (R. Conlon). Analyzing Patterns of Alternative Splicing (C. Smith, et al.). Index.
Synopsis
Here is the most complete guide available to the isolation, analysis, and synthesis of RNA. It covers everything researchers and laboratory workers need to know about the study of gene expression via RNA analysis-from the theory behind the methods, to actual problem-solving techniques., This work covers RNA experimental procedures, from isolation of RNA in a variety of cell types and tissues, to the large and small scale synthesis of RNA. It establishes experimental procedures such as RNA blotting and nuclease protection. In addition, this guide contains protocols for the techniques of quantitative PCR, differential display and the use of non-radioactive probes for RNA detection., Here is the most complete guide available to the isolation, analysis, and synthesis of RNA. It covers everything researchers and laboratory workers need to know about the study of gene expression via RNA analysis-from the theory behind the methods, to actual problem-solving techniques. Step-by-step protocols are presented for each method. A careful presentation of the experimental formalities of these protocols enables specialists and nonspecialists alike to implement the methods easily in the laboratory. Each protocol is accompanied by the theoretical background underlying the experimental procedure and most chapters contain illustrations of typical results and troubleshooting tips. A Laboratory Guide to RNA offers a straightforward detailed account of experimental procedures, ranging from the isolation of RNA from a variety of cell and tissue types, detection analysis, and quantitation using a range of strategies, to large- and small-scale synthesis of RNA. This unique guide not only covers established procedures such as RNA blotting and nuclease protection, but also the latest protocols for quantitative PCR and differential display. Protocols addressing in situ hybridization are highlighted in an eight-page, full-color section that illustrates the power of the technique for detection of gene expression in tissues and whole organisms. Featuring contributions from leading research laboratories and the biotechnology field, A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis provides all the methods required for RNA analysis. It is the ideal laboratory guide for research scientists, graduate students, and lab personnel who need a solid reference on the analysis of gene expression at the RNA level.
LC Classification Number
QP623.L33 1996

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