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Microinjection : Methods and Protocols, Hardcover by Liu, Chengyu (EDT); Du, ...

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Item specifics

Condition
Like New: A book in excellent condition. Cover is shiny and undamaged, and the dust jacket is ...
Book Title
Microinjection : Methods and Protocols
ISBN
9781493988303
Subject Area
Science, Medical
Publication Name
Microinjection : Methods and Protocols
Item Length
10 in
Publisher
Springer New York
Subject
Biotechnology, Chemotherapy, Genetics
Series
Methods in Molecular Biology Ser.
Publication Year
2018
Type
Textbook
Format
Hardcover
Language
English
Author
Yubin Du
Item Width
7 in
Item Weight
44 Oz
Number of Pages
Xiv, 540 Pages

About this product

Product Information

1. Microinjection and Micromanipulation: A Historical Perspective Wenhao Xu 2. Production of Transgenic Mice by Pronuclear Microinjection Xin-an Pu, Anthony P. Young, and H. Michael Kubisch 3. Transgene Recombineering in Bacterial Artificial Chromosomes Michael G. Zeidler and Thomas L. Saunders 4. Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice Ruby Yanru Chen-Tsai 5. Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method Takuya Sato and Takehiko Ogawa 6. Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer Yubin Du, Wen Xie, Fan Zhang, and Chengyu Liu 7. Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection Pinghu Liu, Yan Li, Jingqi Lei, and Lijin Dong 8. Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, and Colin L. Sweeney 9. Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique Melissa A. Scott and Yueh-Chiang Hu 10. Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation Wenning Qin and Haoyi Wang 11. Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides Ye Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, and Chengyu Liu 12. Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama 13. The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications Naomi Nakagata, Jorge Sztein, and Toru Takeo 14. The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm Toru Takeo, Jorge Sztein, and Naomi Nakagata 15. Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, and Takayuki Shindo 16. Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology Tuija M. Alcantar and Xin Y. Rairdan 17. Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos &nbs

Product Identifiers

Publisher
Springer New York
ISBN-10
1493988301
ISBN-13
9781493988303
eBay Product ID (ePID)
17038746789

Product Key Features

Author
Yubin Du
Publication Name
Microinjection : Methods and Protocols
Format
Hardcover
Language
English
Subject
Biotechnology, Chemotherapy, Genetics
Series
Methods in Molecular Biology Ser.
Publication Year
2018
Type
Textbook
Subject Area
Science, Medical
Number of Pages
Xiv, 540 Pages

Dimensions

Item Length
10 in
Item Width
7 in
Item Weight
44 Oz

Additional Product Features

Series Volume Number
1874
Number of Volumes
1 Vol.
Lc Classification Number
Tp248.13-248.65
Table of Content
Microinjection and Micromanipulation: A Historical Perspective.- Production of Transgenic Mice by Pronuclear Microinjection.- Transgene Recombineering in Bacterial Artificial Chromosomes.- Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice.- Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method.- Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer.- Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection.- Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice.- Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique.- Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation.- Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides.- Improvement of Mouse Cloning from Any Type of Cellby Nuclear Injection.- The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications.- The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm.- Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line.- Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology.- Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos.- Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells.- Generation of Rabbit Models by Gene Editing Nucleases.- Production of Genetically Engineered Porcine Embryos by Handmade Cloning.- Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production.- Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer.- Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting.- Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes.- Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing.- Microinjection in Zebrafish for Genome Editing and Functional Studies.- Microinjection of Marine Fish Eggs.- Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method.- Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus.- Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function.
Copyright Date
2019
Illustrated
Yes

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