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Lipid Signaling Protocols by Mark Waugh (English) Paperback Book
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eBay item number:386711209774
Item specifics
- Condition
- Brand New: A new, unread, unused book in perfect condition with no missing or damaged pages. See all condition definitionsopens in a new window or tab
- ISBN-13
- 9781493950072
- Type
- NA
- Publication Name
- NA
- ISBN
- 9781493950072
- Publication Year
- 2016
- Format
- Trade Paperback
- Language
- English
- Book Title
- Lipid Signaling Protocols
- Item Length
- 10in
- Publisher
- Springer New York
- Genre
- Science
- Topic
- Life Sciences / Cell Biology, Life Sciences / Biochemistry
- Item Width
- 7in
- Item Weight
- 182.1 Oz
- Number of Pages
- Xi, 257 Pages
About this product
Product Information
In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Product Identifiers
Publisher
Springer New York
ISBN-10
149395007x
ISBN-13
9781493950072
eBay Product ID (ePID)
12038375254
Product Key Features
Book Title
Lipid Signaling Protocols
Format
Trade Paperback
Language
English
Topic
Life Sciences / Cell Biology, Life Sciences / Biochemistry
Publication Year
2016
Genre
Science
Number of Pages
Xi, 257 Pages
Dimensions
Item Length
10in
Item Width
7in
Item Weight
182.1 Oz
Additional Product Features
Series Volume Number
1376
Number of Volumes
1 Vol.
Lc Classification Number
Qh573-671
Edition Number
2
Table of Content
Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format.- Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass Spectrometry.- Fluorescent Assays for Ceramide Synthase Activity.- Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II Alpha.- Measurement of Long Chain Fatty Acyl-CoA synthetase Activity.- Qualitative and Quantitative in vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specificity.- Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity.- Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microdomains.- Fluorescence Recovery after Photobleaching Analysis of the Diffusional Mobility of Plasma Membrane Proteins: HER3 Mobility in Breast Cancer Cell Membranes.- Isolation and Analysis of Detergent-Resistant Membrane Fractions.- Detection of Isolated Mitochondria-Associated ER Membranes using the Sigma-1 Receptor.- Using Surface Plasmon Resonance to Quantitatively Assess Lipid-Protein Interactions.- Analyzing Protein-phosphoinositide Interactions with Liposome Flotation Assays.- High-Throughput Flurometric Assay for Membrane-Protein Interaction.- Guidelines for the use of Protein Domains in Acidic Phospholipid Imaging.- Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]serine.- Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells.- Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions.- Single-molecule Imaging of Signal Transduction via GPI-anchored Receptors.- Measuring Phosphatidylinositol Generation on Biological Membranes.- Assay for CDP-diacylglycerol Generation by CDS in Membrane Fractions.
Copyright Date
2016
Dewey Decimal
547.77
Series
Methods in Molecular Biology Ser.
Dewey Edition
23
Illustrated
Yes
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