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Heterologous Expression of Membrane Proteins: Methods and Protocols: New
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eBay item number:364024166304
Item specifics
- Condition
- Brand New: A new, unread, unused book in perfect condition with no missing or damaged pages. See all condition definitionsopens in a new window or tab
- Book Title
- Heterologous Expression of Membrane Proteins: Methods and Protoco
- Publication Date
- 2009-12-18
- Pages
- 272
- ISBN
- 9781607613435
About this product
Product Identifiers
Publisher
Humana Press
ISBN-10
1607613433
ISBN-13
9781607613435
eBay Product ID (ePID)
77404536
Product Key Features
Number of Pages
Xi, 272 Pages
Language
English
Publication Name
Heterologous Expression of Membrane Proteins : Methods and Protocols
Subject
Life Sciences / Molecular Biology, Life Sciences / Cell Biology, Life Sciences / Biochemistry, Life Sciences / Biology, Chemistry / Organic
Publication Year
2009
Type
Textbook
Subject Area
Science
Series
Methods in Molecular Biology Ser.
Format
Hardcover
Dimensions
Item Weight
28.3 Oz
Item Length
10 in
Item Width
7 in
Additional Product Features
Intended Audience
Scholarly & Professional
Reviews
From the reviews:Well-illustrated book provides an overview of different heterologous expression systems available to produce membrane proteins for structural analysis to facilitate understanding of how these proteins function and how their functions can be modified by drug treatment. … Written for students and experienced researchers, the book also will be useful for clinicians interested in the care of patients with cancer … . provides valuable information on membrane proteins for novice and experienced researchers. Clinicians will find it extremely useful for developing methods and protocols for research. (Omer Iqbal, Doody's Review Service, July, 2010), From the reviews: "Well-illustrated book provides an overview of different heterologous expression systems available to produce membrane proteins for structural analysis to facilitate understanding of how these proteins function and how their functions can be modified by drug treatment. ... Written for students and experienced researchers, the book also will be useful for clinicians interested in the care of patients with cancer ... . provides valuable information on membrane proteins for novice and experienced researchers. Clinicians will find it extremely useful for developing methods and protocols for research." (Omer Iqbal, Doody's Review Service, July, 2010)
Dewey Edition
22
Series Volume Number
601
Number of Volumes
1 vol.
Illustrated
Yes
Dewey Decimal
572.694
Table Of Content
Heterologous Expression of Membrane Proteins for Structural Analysis.- Production of Membrane Proteins in Escherichia coli and Lactococcus lactis.- Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli.- Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies.- Membrane Protein Expression in Lactococcus lactis.- Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae.- Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells.- Expression of Membrane Proteins in Drosophila Melanogaster S2 Cells: Production and Analysis of a EGFP-Fused G Protein-Coupled Receptor as a Model.- Membrane Protein Expression in the Eyes of Transgenic Flies.- Expression of Mammalian Membrane Proteins in Mammalian Cells Using Semliki Forest Virus Vectors.- Membrane Protein Expression in Cell-Free Systems.- Practical Considerations of Membrane Protein Instability during Purification and Crystallisation.- Membrane Protein Solubilization.- Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents for Studying Membrane Proteins In vitro.- Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sarcoplasmic Reticulum Ca2+-ATPase.
Synopsis
This book provides an overview of the different heterologous expression systems available to produce various membrane proteins for structural analysis. Chapters examine membrane protein solubilization, purification, and instability in solution., Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols , expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols , is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field., Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.
LC Classification Number
QD431-431.7
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Seller feedback (515,221)
- w***p (432)- Feedback left by buyer.Past monthVerified purchaseItem as described. Fast shipping. A++
- t***i (640)- Feedback left by buyer.Past monthVerified purchaseEVERYTHING WAS DONE QUICKLY AND FAIR AND PROMPTLY, AMEN
- n***d (1131)- Feedback left by buyer.Past monthVerified purchaseI am very happy with this