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Heterologous Protein Production in Cho Cells : Methods and Protocols, Hardcov...
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Located in: Jessup, Maryland, United States
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eBay item number:353751458259
Item specifics
- Condition
- Brand New: A new, unread, unused book in perfect condition with no missing or damaged pages. See all condition definitionsopens in a new window or tab
- ISBN
- 9781493969715
- Publication Year
- 2017
- Format
- Hardcover
- Language
- English
- Book Title
- Heterologous Protein Production in Cho Cells : Methods and Protocols
- Item Length
- 10in
- Publisher
- Springer New York
- Genre
- Science
- Topic
- Life Sciences / Molecular Biology, Life Sciences / Cell Biology, Life Sciences / Biochemistry
- Item Width
- 7in
- Item Weight
- 32.3 Oz
- Number of Pages
- X, 252 Pages
About this product
Product Information
This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using 'omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.
Product Identifiers
Publisher
Springer New York
ISBN-10
1493969714
ISBN-13
9781493969715
eBay Product ID (ePID)
237618934
Product Key Features
Book Title
Heterologous Protein Production in Cho Cells : Methods and Protocols
Format
Hardcover
Language
English
Topic
Life Sciences / Molecular Biology, Life Sciences / Cell Biology, Life Sciences / Biochemistry
Publication Year
2017
Genre
Science
Number of Pages
X, 252 Pages
Dimensions
Item Length
10in
Item Width
7in
Item Weight
32.3 Oz
Additional Product Features
Series Volume Number
1603
Number of Volumes
1 Vol.
Lc Classification Number
Qd431-431.7
Table of Content
Strategies and Considerations for Improving Expression of 'Difficult to Express' Proteins in CHO Cells.- Glycoengineering of CHO Cells to Improve Product Quality.- Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension.- Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression.- Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells.- Conditional Knockdown of Endogenous MicroRNAs in CHO Cells using TET-ON-SanDI Sponge Vectors.- Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells.- Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture.- Selection of High-Producing Clones using FACS for CHO Cell Line Development.- The 'Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity.- A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data.- Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells.- Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells.- Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture.- Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells.- Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses.
Copyright Date
2017
Lccn
2017-935545
Series
Methods in Molecular Biology Ser.
Illustrated
Yes
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eBay item number:353751458259
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