Picture 1 of 1
Gallery
Picture 1 of 1
Have one to sell?
1999 Spiral Bound Molecular Biology Techniques: An Intensive Laboratory Course
US $61.90
ApproximatelyS$ 79.52
or Best Offer
Condition:
“Pages are crisp and clean, no marking. Cover is good. Binding is tight/good. ex-library book with ”... Read moreabout condition
Good
A book that has been read but is in good condition. Very minimal damage to the cover including scuff marks, but no holes or tears. The dust jacket for hard covers may not be included. Binding has minimal wear. The majority of pages are undamaged with minimal creasing or tearing, minimal pencil underlining of text, no highlighting of text, no writing in margins. No missing pages.
Oops! Looks like we're having trouble connecting to our server.
Refresh your browser window to try again.
Shipping:
US $4.47 (approx S$ 5.74) USPS Media MailTM.
Located in: San Jose, California, United States
Delivery:
Estimated between Wed, 24 Sep and Fri, 26 Sep to 94104
Returns:
30 days return. Buyer pays for return shipping. If you use an eBay shipping label, it will be deducted from your refund amount.
Coverage:
Read item description or contact seller for details. See all detailsSee all details on coverage
(Not eligible for eBay purchase protection programmes)
Seller assumes all responsibility for this listing.
eBay item number:297604735062
Item specifics
- Condition
- Good
- Seller Notes
- ISBN
- 9780125839907
About this product
Product Identifiers
Publisher
Elsevier Science & Technology
ISBN-10
0125839901
ISBN-13
9780125839907
eBay Product ID (ePID)
289265
Product Key Features
Number of Pages
234 Pages
Publication Name
Molecular Biology Techniques : an Intensive Laboratory Course
Language
English
Publication Year
1998
Subject
Life Sciences / Molecular Biology
Type
Textbook
Subject Area
Science
Format
Trade Paperback
Dimensions
Item Height
0.2 in
Item Weight
14.4 Oz
Item Length
9.3 in
Item Width
6.5 in
Additional Product Features
Intended Audience
Scholarly & Professional
Dewey Edition
21
Illustrated
Yes
Dewey Decimal
572.8
Table Of Content
Preface. Course Synopsis: Introduction.Safety Precautions.Daily Schedule.Acknowledgements. Exercises: I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning: Cesium chloride-ethidium bromide density gradient centrifugation.PCR to synthesize virD2 flanked with restriction sites.Restriction digests of plasmid pGEX2 and PCR products.Purification of DNA fragments from agarose.Ligation of PCR product to pGEX2 vector. Transformation of E. coli with the ligated plasmid.Small-scale preparation of plasmid DNA by the alkaline lysis method.Restriction analysis.Study questions. Protein Expression, Purification, and Analysis: Expression and purification of a fusion protein.SDS-polyacrylamide gel electrophoresis.Silver stain detection of proteins.Study questions. Oligonucleotide-Directed Mutagenesis: Restriction digests of virD2 (in pCS64) and pUC119.Purification of DNA fragments from agarose.Ligatation of restriction fragment and vector.Transformation of E. coli with the ligated plasmid and recovery clones.Small-scale preparation of plasmid DNA from broth cultures.Restriction digest of DNA: examination to confirm insert.Preparation of single-stranded DNA template.Phosphorylation of oligonucleotide.Annealing mutant oligonuleotide to template. In vitro DNA synthesis by primer extension.Transform synthesis reaction into E. coli DH5a. Small-scale preparation of plasmid DNA.Confirmation of mutants by restriction analysis.Study questions. DNA Sequencing: Polyacrylamide sequencing gel electrophoresis.Didexy sequencing.Automated Sequencing.Introduction to databases and gene sequence analysis.Study questions. Southern Blot Detection of DNA: Preparation of genomic DNA from Agrobacterium tumefaciens .Restriction digestion of genomic DNA.Agarose gel electrophoresis of restriction fragments.Southern blot: denaturation and blotting of DNA gel.Preparation of probe nick translation.Hybridization and washing of Southern blots.Study questions. Northern Blot Detection of mRNA: Preparation of RNA from tobacco leaves.Agarose-formaldehyde gel electrophoresis.Northern blot: denaturation and blotting of RNA.Probe preparation. Hybridization and washing of Northern blots.Study questions. Protein Interaction Analysis in Yeast: Yeast transformation.Filter b-galactosidase assay.Index.
Synopsis
This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Provides well-tested experimental protocols for each technique Lists the reagents and preparation of each experiment separately Contains a complete schedule of experiments and the preparation required Includes study questions at the end of each chapter, This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression.
LC Classification Number
QH506
Item description from the seller
Seller feedback (1,687)
- k***_ (25)- Feedback left by buyer.Past monthVerified purchaseE erything went well. Would buy again.
- eBay 自動留下信用評價- Feedback left by buyer.Past month訂單成功完成 — 物品享追蹤服務且準時送達
- eBay 自動留下信用評價- Feedback left by buyer.Past month訂單成功完成 — 物品享追蹤服務且準時送達
Seller feedback (1,687)
- k***_ (25)- Feedback left by buyer.Past monthVerified purchaseE erything went well. Would buy again.
- eBay 自動留下信用評價- Feedback left by buyer.Past month訂單成功完成 — 物品享追蹤服務且準時送達
- eBay 自動留下信用評價- Feedback left by buyer.Past month訂單成功完成 — 物品享追蹤服務且準時送達